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Division of Chemical Toxicology Fundamentals Workshop
Saturday, August 21, 2010
Location TBD
Workshop Faculty
Nancy E. Hopkins, PhD; Department of Cell and Molecular Biology, Tulane University; nhopkin@tulane.edu
Matthew Hutzler, PhD; Boehringer-Ingelheim Corporation; matt.hutzler@boehringer-ingelheim.com
Peter C. Dedon, MD, PhD; Department of Biological Engineering, MIT; pcdedon@mit.edu
Summary
The goal of this 4-hour educational workshop is to provide students, postdoctoral scientists, and younger academic and industry scientists with the fundamental theory and practice of modern chromatography and mass spectrometry, with applications in drug development and toxicology.
Schedule
| 1:00 |
Introduction and overview - Nancy Hopkins |
| 1:10 |
Fundamentals of chromatography - Matt Hutzler
- drivers : solid phase extraction, GC, HPLC, UPLC;
- resolvers : reverse phase, normal phase, ion exchange, HILIC;
- detectors : UV, mass spec, fluorescence, scintillation;
- chromatographic theory : HETP, resolution, efficiency, etc.;
- principles in practice : illustrations with small molecules and peptides.
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| 1:50 |
Fundamentals of mass spectrometry - Peter Dedon
- ionization sources : ESI, EI, chemical ionization, MALDI;
- mass analyzers : single quad, triple quad, ion trap, TOF;
- signal enhancers ; the mass spectrum; isotopes; accuracy and resolution;
- principles in practice : peptides, small molecules to illustrate concepts.
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| 2:50 |
Break
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| 3:00 |
Applications in toxicology - Peter Dedon
- Biomarkers (single molecules) - DNA, protein, lipid damage products
- Metabolomics (multiple molecules) - serum and urine
- Proteomics - simple protein identification, phosphoproteomics
- Sample preparation issues , artifacts (e.g., 8-oxoG quantification)
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| 4:00 |
Applications in drug development - Matt Hutzler
- LC/MS methods to support Metabolite Profiling/Identification
- State-of-the-art technology : OrbiTrap high resolution MS, mass-defect-filtering (MDF)
- Rapid LC/MS assays to support high-throughput screening (HTS) efforts in drug discovery
- Instrumentation that enables HTS (e.g. UPLC technology, RapidFire technology)
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| 5:00 |
Discussion/Q&A and Networking Reception - Nancy Hopkins
All TOXI members are invited to this reception-no need to register for the workshop. |
Registration
Register for this Workshop when you register for the ACS meeting.
Fees
The cost is free to TOXI members and $15.00 for nonmembers.
If you have already registered for the meeting, you can add the workshop to your ticketed events accessing your ACS Meeting Resigtration at the XPress Toolkit: https://www.xpressreg.net/register/acsf080/XPressToolkit/
Find the link to "Add Social Event Tickets or an Abstract CD-ROM". In order to receive your ticket(s) with your badge, you must update your record by July 9. Tickets purchased after July 9 must be picked up on site.
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